ABSTRACT
The objective of this study was to determine the levels of TERT mRNA and TERT protein expression in stomach precancerous lesions such as intestinal metaplasia (IM) and gastric ulcer (GU) and compare them to gastric cancer (GC). Real-time PCR was performed to detect TERT mRNA expression levels in 35 biopsies of IM, 30 of GU, and 22 of GC and their respective normal mucosas. TERT protein was detected by immunohistochemistry in 68 samples, 34 of IM, 23 of GU, and 11 of GC. Increased TERT mRNA expression levels were observed in a significant number of cases, i.e., 46 percent of IM, 50 percent of GU, and 79 percent of GC. The relative mean level of TERT mRNA after normalization with the β-actin reference gene and comparison with the respective adjacent normal mucosa was slightly increased in the IM and GU groups, 2.008 ± 2.605 and 2.730 ± 4.120, respectively, but high TERT mRNA expression was observed in the GC group (17.271 ± 33.852). However, there were no statistically significant differences between the three groups. TERT protein-positive immunostaining was observed in 38 percent of IM, 39 percent of GU, and 55 percent of GC. No association of TERT mRNA and protein expression with Helicobacter pylori infection or other clinicopathological variables was demonstrable, except for the incomplete type vs the complete type of IM. This study confirms previous data of the high expression of both TERT mRNA and protein in gastric cancer and also demonstrates this type of changed expression in IM and GU, thus suggesting that TERT expression may be deregulated in precursor lesions that participate in the early stages of gastric carcinogenesis.
Subject(s)
Humans , Middle Aged , Precancerous Conditions/metabolism , RNA, Messenger/analysis , Stomach Neoplasms/metabolism , Stomach Ulcer/metabolism , Telomerase/analysis , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Helicobacter pylori , Helicobacter Infections/metabolism , Helicobacter Infections/pathology , Immunohistochemistry , Intestines/pathology , Metaplasia/metabolism , Neoplasm Proteins/metabolism , Precancerous Conditions/pathology , Real-Time Polymerase Chain Reaction , Stomach Neoplasms/pathology , Stomach Ulcer/pathology , Telomerase/geneticsABSTRACT
Investigation for prevalence of antibodies to hepatitis C virus (HCV) and to Treponema pallidum was conducted in 883 females with gynecologic disorders who were admitted to the gynecological ward of the Department of Obstetrics and Gynecology, Siriraj Hospital during April to August 1991. The study population consisted of 678 patients with malignancies and 205 patients with benign diseases. Anti-HCV antibody was found in 3.1% of the cases with malignancies and 1.46% of those with benign diseases. Among the gynecologic malignant group, the patients with carcinoma of cervix had the highest prevalence of HCV antibody (3.6%). The positive serologic tests for syphilis in patients with carcinoma of cervix (9.8%) were significantly higher from those in patients with ovarian carcinoma (3.75%) (p < 0.01). There were 3 cases with carcinoma of cervix who were simultaneously sero-positive for both HCV and syphilis.